Abstract:

The objective of this study were to optimize a set of microsatellite markers in Simmental paternity testing, establish an accurate, efficient and economic paternity testing system, and provide a scientific basis for genetic evaluation and breeding of Simmental. Based on fluorescent primer and automatic sequencing techniques, genetic polymorphisms of 11 microsatellite markers were genotyped in 107 Simmental from Mongolia Ulgai. Finally 8 loci were selected as core loci to establish paternity testing system of Simmental, and paternity testing power was detected in the validation group of 10 fathers-sons and 1 no related father by likelihood-based methods. The result showed that the average of allele number, observed heterozygosity, expected heterozygosity and polymorphism information content were 11.4, 0.804, 0.828 and 0.807, respectively. Their cumulative exclusion probability reached 0.99, 0.999 5 and 0.999 998 on three different occasions. The assignment result of validation group was consistent with pedigree records and LOD varied from 9.29 to 14.66 with 95% confidence. This set of STRs have a high genetic polymorphism and an excellent value in paternity testing of Simmental, which can validate paternity efficiently with a lower cost.